Abstract
AbstractA bacterial strain, designated Aeromonas sp. kumar, was isolated from a water sample collected from Red tide Bloom occurred in the region of Gulf of Mannar region, Puthumadam Coast, India and the strain was identified using 16S rRNA based identification. During the sample collection, microbiology analysis was done to study the morphology of the bacteria. Pure culture of strain was maintained through out the study. DNA was isolated and sequenced using 16S rRNA primers. A length of 1452 nucleotide was sequenced and was put in public data base for obtaining accession number. The sequence was studied using MEGA 4, to estimate the evolutionary distances and to construct the Phylogenetic tree. Along with that Regulatory elements and Transcription factors were studied using BPROM tool. In genetics, a promoter is a region of DNA that facilitates the transcription of a particular gene. Promoters are typically located near the genes they regulate, on the same strand and upstream (towards the 5' region of the sense strand). The objective of the study is to predict the regulatory elements which are -10 box, -35box and three Transcription Factors (rpoD19, rpoD17 and araC) with their binding sites in the 16S rRNA gene of Aeromonas sp. kumar. The gene bank accession number for 16S rRNA gene of Aeromonas sp. kumar is FJ896014.
Highlights
In the present research communication, we report the genotypic characterization of Aeromonas sp. kumar based on phylogenetic analysis and to identify their regulatory elements
All along with the DNA sequence of Aeromonas sp. kumar 16S rRNA (Accession No FJ896014) gene from the Genbank as an input sequence for the identification of regulatory elements analysis because this strain has maximum similarity (97% identity) during BLAST13
In the present study genomic DNA was extracted from the strain and 16S rRNA sequence was done and a total of 1453bp of 16S rRNA was obtained
Summary
In the present study genomic DNA was extracted from the strain and 16S rRNA sequence was done and a total of 1453bp of 16S rRNA was obtained. 2. In conclusion, the r-RNA based analysis is a central method in microbiology used to explore the microbial diversity and used as a method for bacterial identification.
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