Abstract
Pea weevil (Bruchus pisorum) is a damaging insect pest affecting pea (Pisum sativum) production worldwide. No resistant cultivars are available, although some levels of incomplete resistance have been identified in Pisum germplasm. To decipher the genetic control underlying the resistance previously identify in P. sativum ssp. syriacum, a recombinant inbred line (RIL F8:9) population was developed. The RIL was genotyped through Diversity Arrays Technology PL’s DArTseq platform and screened under field conditions for weevil seed infestation and larval development along 5 environments. A newly integrated genetic linkage map was generated with a subset of 6,540 markers, assembled into seven linkage groups, equivalent to the number of haploid pea chromosomes. An accumulated distance of 2,503 cM was covered with an average density of 2.61 markers cM−1. The linkage map allowed the identification of three QTLs associated to reduced seed infestation along LGs I, II and IV. In addition, a QTL for reduced larval development was also identified in LGIV. Expression of these QTLs varied with the environment, being particularly interesting QTL BpSI.III that was detected in most of the environments studied. This high-saturated pea genetic map has also allowed the identification of seven potential candidate genes co-located with QTLs for marker-assisted selection, providing an opportunity for breeders to generate effective and sustainable strategies for weevil control.
Highlights
Pea (Pisum sativum L.) is the second most cultivated temperate grain legume in the world and the first in Europe[1]
This recently constructed integrated genetic linkage map developed with an advanced recombinant inbred line (RIL) population (F8:9) from an interspecific cross (P. sativum ssp. syriacum × P. sativum ssp. sativum) and genotyped by DArTseq technology consists of seven linkage groups (LGs) covering a total length of 2,503.6 cM, with an average concentration of 2.61 markers cM−1 and an average marker-marker gap distance of 1.49 cM
6,447 DArTseq-derived markers were polymorphic on the panel of 108 RIL populations, showing a good polymorphic information content (PIC) average value of 0.44, which indicates that those markers should be considered of importance[20]
Summary
Pea (Pisum sativum L.) is the second most cultivated temperate grain legume in the world and the first in Europe[1]. (mm) to be a combination of antixenosis and antibiosis mechanisms resulting in reduced seed infestation and retarded larval development[15] This accession was previously identified as resistant to a number of other stresses such as ascochyta blight (Didymella pinodes)[16], broomrape (Orobanche crenata)[17], pea aphid (Acyrtosiphon pisum)[15], and drought[18], and it was early introduced in the crossing program and extensively used in the breeding program at the Institute for Sustainable Agriculture, CSIC (Córdoba, Spain). In order to achieve reliable information regarding the genomic regions involved in resistance, the precision in trait scoring and the availability of high density genetic maps are crucial In this scenario, the Diversity Arrays Technology (DArT) in combination with next-generation sequencing platforms[20,21] known as DArTseqTM, provides a good choice as a high throughput marker genotyping platform that can develop a relatively large number of polymorphic markers to build dense genetic maps with low-cost investments[22]. Messire) RIL F8:9 population, as well as the identification of regions in the pea genome controlling Bp seed infestation and larval development
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