Abstract
Pseudomonas fluorescens not only enhances the plant growth but also controls the fungal pathogens by production of anti fungal metabolites. The objective of this experiment was to identify P. fluorescens using different biochemical tests. This research was carried out in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal. The result of this experiment indicated that P. fluorescens gave positive result for Catalase test, Gelatin liquefaction, Fluorescent pigment and Oxidase test but negative result for starch hydrolysis test. The colony of P. fluorescens was maximum in maize seed than that of rice seed after two hours of inoculation, whereas higher number of colony was found in rice seed than that of maize seed after twenty four hours of seed inoculation. This findings is useful for identifying colony of P. fluorescens per seed which is necessary for better seedling growth and effective biological control of pathogens.
Highlights
Pseudomonas species encompasses a group of common rod shape, gram negative, one or more polar flagella providing mortality, aerobic in nature, non-spore farming, positive catalase test, positive oxidase test, nonpathogenic saprophytes that colonize soil, water and plant surface environments
Experimental Location and Preparation This experiment was conducted in Plant Pathology Laboratory at Agriculture and Forestry University (AFU), Rampur, Chitwan, Nepal in October 03, 2018
The entire test showed the presence of Pseudomonas fluorescens in the liquid
Summary
Pseudomonas species encompasses a group of common rod shape, gram negative, one or more polar flagella providing mortality, aerobic in nature, non-spore farming, positive catalase test, positive oxidase test, nonpathogenic saprophytes that colonize soil, water and plant surface environments. It secretes pyoverdine a fluorescent yellow green siderophore under iron limiting condition. Pseudomonas fluorescent produces additional type of siderophore such as thioquindobactin (Scales et al, 2014) It is an obligate aerobe, except for some strains that can utilize NO3 as an electron acceptor in place of O2. Pseudomonas fluorescens has simple nutritional requirements and grows well in mineral salts media supplemented with any of a large number of carbon sources
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