Abstract
Objective To find the development-related genes of Plasmodium yoelii(P.yoelii)during the exo-erythroeytie stage by sequence analysis and predict their functions.nethods Total RNA was extraeted from the liver of normal rats and infected rats followed by differential display RT-PCR(DD-PCR)with the primer containing 40% C+C.The primer designed and synthesized accommodates the high adenine and thymidine rich genome of P.yoelii.The special bands of the PCR products were purified and cloned into plasmid Tvector for sequencing,and then identified by Blast to find the homology sequences.Results Six genes,named PyHs4、PyHs5、PyHs6、PyHs7、PyHs8、PyHs9,were found in relation to the development of P.yoelii during exo-erythroeytic stage.According to Blastn analysis,in these genes,91% sequences of PyHs4 were identical to phosphoacetylglucosamine mutase (AGM,PY02130) gene,and PyHs5 were exactly the same gene encoding erythrocyte memberance protein 3 (EMP3,PY05500).The sequences of PyHs6、PyHs7、eyHs8、PyHs9 were supposed to be hypothetical genes of malaria parasite with unknown functions.Conclusions The developmental genes of P.yoelii during exo-erythrocytic stage have been successfully amplified by DD-PCR technique thus to lay the foundation for the research on expression of the recombinant and development of new vaccine. Key words: Plasmodium yoelii; Differential display reverse transcriptase PCR; Exo-erythrocytic stage; Differential gene
Published Version
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