Abstract
An on-line HPLC-electrospray ionization tandem mass spectrometry system has been developed for the separation and characterization of the photochemical oxidation products of 5,10,15,20-tetra( m-hydroxyphenyl)chlorin ( m-THPC), a new photodynamic therapeutic agent. m-THPC was photo-oxidized in methanol solution by exposure to white laboratory light for one week. The products were then separated by isocratic elution on an Apex C 18 column with 0.1% trifluoroacetic acid in acetonitrile (23:77, v/v) as mobile phase and characterized on-line by electrospray tandem mass spectrometry. The results showed the formation of 5,10,15,20-tetra( m-hydroxyphenyl) porphyrin ( m-THPP), hydroxy m-THPP and three hydroxy m-THPC isomers. Hydroxylation on the reduced pyrrole ring of m-THPC was the most prominent reaction.
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