Abstract
AbstractThe present work describes a liquid chromatography‐electrospray ionization mass spectrometry (LC‐ESI‐MS) method for rapid identification of phenylethanoid glycosides in plant extract from Plantago asiatica L. By using a binary mobile phase system consisting of 0.2% acetic acid and acetonitrile under gradient conditions, a good separation was achieved on a reversed‐phase C18 column. The [M–H]− ions, the molecular weights, and the fragment ions of phenylethanoid glycosides were obtained in the negative ion mode using LC‐ESI‐MS. The identification of the phenylethanoid glycosides (peaks 1–3) in the extract of P. asiatica L. was based on matching their retention time, the detection of molecular ions, and the fragment ions obtained by collision‐induced dissociation (CID) experiments with those of the authentic standards and data reported in the literature.
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