Abstract
A general method for the identification of peptides containing tyrosine, tryptophan, phenylalanine and cysteine and a preliminary study of the identification of peptides containing monoiodotyrosine, diiodotyrosine, triiodothyronine and thyroxine by high-performance liquid chromatography photodiode-array ultraviolet—visible detection is reported. The technique was tested with an immunoglobulin light chain and with an in vitro iodinated urinar human complex-forming glycoprotein, heterogeneous in charge (protein HC), and human thyroglobulin (Tg) after enzymatic digestion. The system continuously monitors wavelengths and collects data that can be analysed by comparison with standard spectra via software routines. This procedure saves sample, time and reagents and avoids the use of radioactive reagents.
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