Abstract
Various food-producing animals have been recognized in recent years as a potential reservoir for the spread of antibiotic resistant bacteria that may pose a risk to human health and therefore their dissemination in the food production chain needs to be assessed. In this study, 450 boot swabs from chicken farms were analyzed for the presence of antimicrobial resistance with a focus on β-lactams resistance in Acinetobacter species. Two β-lactamase-encoding genes were first time identified in Acinetobacter lwoffii and Acinetobacter schindleri isolates. The deduced amino acid sequence of OXA-496 shared 93.8% identity with OXA-363. The second OXA-134-like enzyme, OXA-537, had the highest sequence identity (97.8%) with OXA-235 and OXA-237. The results of this study illustrate the occurrence of new OXA-134-like β-lactamases, called OXA-496 and OXA-537, carrying strains of Acinetobacter lwoffii and Acinetobacter schindleri in chicken farm litter, and highlight the possible role of Acinetobacter as a reservoir of resistance genes.
Highlights
Over the last decade, the prevalence of Acinetobacter spp. that are resistant to a number of commonly used antibiotics has increased
Little data is available about the presence of intrinsic and/or acquired β-lactamases producing Acinetobacter spp. in foodproducing animals; intrinsic blaOXA-51-like genes from Acinetobacter baumannii in cattle and pig in Scotland, NDM-1-producing A. baumannii in pig in China and OXA-23-producing Acinetobacter species from horses and cattle have been detected[4,5,6,11], OXA-23-like genes are intrinsic to Acinetobacter radioresistens[12]
A total of 55 Gram-negative bacilli were obtained from selective agar plates, from which 2 (3.6%), 3 (5.5%), 4 (7.3%), 6 (10.9%) and 9 (16.4%) isolates were identified as A. radioresistens, Acinetobacter pittii, Acinetobacter calcoaceticus, A. lwoffii and A. schindleri, respectively
Summary
The prevalence of Acinetobacter spp. that are resistant to a number of commonly used antibiotics has increased. The occurrence of blaNDM-1-producing Acinetobacter lwoffii isolated from chickens in China has been reported[2]. Acquired extended-spectrum β-lactamases (ESBL), CTX-M-15 and TEM-116, have been reported in environmental Acinetobacter spp.[13]. This highlights the propensity of Acinetobacter spp. to acquire different antibiotic resistance determinants. The results of this study illustrate the occurrence of new OXA-134-like β-lactamases, called OXA-496 and OXA-537, carrying strains of Acinetobacter lwoffii and Acinetobacter schindleri in chicken farm litter, and highlight the possible role of Acinetobacter as a reservoir of resistance genes
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