Abstract
The study was carried out to identify muscle protein patterns and gut protease activity of rohu (Labeo rohita), catla (Catla catla), climbing perch (Anabas testudineus) and spotted snakehead (Channa punctatus). Twelve percent Polyacryleamide substrate SDS-PAGE was done to visualize the activity of the enzyme after casein hydrolysis and protein pattern of the species. Three alkaline proteases of different molecular weight were observed in rohu, two in catla, five in climbing perch and four in spotted Snakehead from the zymogram. This revealed that the existence of alkaline protein in the alimentary tract of respective species. Fourteen distinct protein bands in rohu, fifteen in catla, sixteen in climbing perch and nineteen in soptted snakehead were visualized from the electrophoretic patterns of fish muscle extract. From the result, it is assumed that heavy molecular weight myosin and low molecular weight actin were prominent in all the experimental species.
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