Identification of molecular species of acylglycerols of Philippine wild edible mushroom, Ganoderma lucidum

Abstract

We identified the lipid species of the solvent extract from liquid-cultured mycelium of G. lucidum NRRL66208. The molecular species of acylglycerols containing all normal fatty acids were identified. One hundred and three molecular species of acylglycerols containing all normal fatty acids were identified by the MS fragmentation involved their constituent fatty acids. The chain lengths of the constituent fatty acids were from 14 to 26 carbon atoms and the numbers of double bonds were from zero to three. The contents of the molecular species of triacylglycerols in the mushroom lipid extract in decreasing order were: OOP (2.45%), OOO (1.94%), LLP (1.92%), OLP (1.80%), LLO (1.57%), OPP (1.30%), OOL (1.09%), OOS (0.88%), and LLL (0.85%). Forty one molecular species of acylglycerols out of 103 contained fatty acids with odd numbered carbon atoms. The fatty acids with odd numbered carbon atoms were 15:0, 17:0, 17:1, 19:0, 19:1, 21:0, 23:0 and 25:0. The total content of fatty acids with odd numbered carbon atoms was about 6.6% of all of the fatty acids of the triacylglycerols containing three normal fatty acids, and contents of individual fatty acids with odd numbered carbon atoms were as: 15:0 (2.76%), 17:0 (2.13%), 17:1 (1.35%), 19:0 (0.20%), 19:1 (0.17%). The contents of fatty acids with odd numbered carbon atoms in mushroom were high compared to those in higher plants. This is the first report on the molecular species of acylglycerols in mushroom.