Identification of molecular marks associated with early flowering in Campomanesia adamantium (Cambess.) O. Berg

Abstract

Campomanesia adamantium is a fruit species of the Cerrado Biome that stands out for having high economic potential and significant genetic variability. Therefore, knowledge on its development and genetic variability is fundamental to define domestication and genetic improvement actions. In this context, identification of molecular marks that may be related to the early flowering of C. adamantium can provide bases for the assisted selection of early plants at nursery level. The objective of this study was to identify molecular marks associated with early flowering in C. adamantium accessions, using the ISSR molecular marker. A total of 17 ISSR primers were used in 46 accessions of C. adamantium (39 early and 07 late). It can be observed in the results for the 46 accessions that the primers produced a total of 104 bands, with a polymorphism of 96%. When analyzing the amplification pattern of early accessions individually, it was possible to observe the amplification of 103 bands, of which 93% were polymorphic. For late accessions, only 87 bands were amplified, of which only 66.67% were polymorphic, showing that these accessions have lower genetic variability than early accessions. Based on the amplification pattern of the primers between early and late accessions, primers 02, 03, 04 and 05 stood out, as they amplified DNA fragments above 70% in early accessions and that were not found in late accessions. It was concluded that there are four molecular marks with strong relationship with early flowering.