Identification of Mirror Repeats in Viral Genomes Using FPCB Analysis

Abstract

The majority of living domains consist of DNA as genetic material with the minor exception of viruses. The uniquenature of every species determines by its unique pattern of genome or gene products. The genomic features become an evidentexample of evolutionary study also. Different types of repeat patterns are observed in genomes of living domains including humanbeings whose two third portion of the genome is repetitive. Among the varied type of repeat sequences Mirror Repeats (MR) playcrucial roles at the genetic level in every species. The major focus of our research is on identification & to check the distributionof mirror repeats in some selected infectious viruses of three different domains (Animal, Plant & Human). For this, we employeda bioinformatics-based approach refer as FASTA PARALLEL COMPLEMENT BLAST (FPCB) to identify unique mirror repeat (MR)sequences. The identified repeats vary in their length as well as found to be distributed throughout the selected viral genomes.The maximum no of MR were reported in the case of Dengue virus (229) & minimum is in the case of TMV (97). In the remainingselected viruses - HCV, HPV, HTLV-1, PVY, Rabies virus 178, 156, 175, 203 & 204 MR sequences were reported. These sequencescan be utilized in many ways like in molecular diagnosis, drug delivery target as well as evolutionary study, etc. The present researchalso helps in the development of novel tools of bioinformatics to study mirror repeats and their functional perspective in thecontext of their occurrence in all domains.