IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS USING TOUCHDOWN PCR AND PHENOTYPIC METHODS FROM PATIENTS AND HOSPITALS ENVIRONMENTS IN DIFFERENT IRAQI CITIES

A M Abd Zaid,N J Kandala

doi:10.36103/ijas.v52i6.1475

Abstract

The study was aimed to evaluate the prevalence of MRSA in some Iraqi hospitals and determine the most powerful methods for identification of MRSA, in order to achieve the, 278 samples were collected from different hospitals in Iraq in various intervals, 204 out of 287 were identified as Staphylococcus aureus by conventional cultural methods and microscopic characteristics and 177 isolates are identified as MRSA by using HiCrome MeReSa Agar Base medium, but 154 of 177 (87%) isolates are methicillin resistance in sensitivity test. MRSA isolates were highly resistant to β-lactam antibiotics and considered multidrug resistant (MDR) in percent of (94.9%). Touchdown PCR used to identify the isolates, 97.05% were identified as Staphylococcus aureus, while 80.88% as MRSA.

Highlights

Human skin and mucosal microbiota contain Staphylococcus aureus which is a common opportunistic pathogen found in human body that causes significant infections [35]
Penicillin-binding protein 2a (PBP 2a) that is coded by gene known as mecA and confer methicillin-resistance in Methicillin resistant Staphylococcus aureus (MRSA) that results in resistance to beta-lactam antibiotics such as Oxacillin, Cloxacillin and Methicillin [16, 20]
MRSA surveillance researches accomplished in community settings are important to best known the molecular and clinical epidemiology of emerging MRSA isolates [33], so, the main objective of current study is to specify the antibiotic susceptibility profiles and MRSA spreads in Iraqi hospital and its link with risk factors in healthcare workers at hospitals, as well as determine the most powerful methods for identification of methicillin resistance S. aureus

Summary

Introduction

Human skin and mucosal microbiota contain Staphylococcus aureus which is a common opportunistic pathogen found in human body that causes significant infections [35]. ‫تشخيص المكو ارت العنقودية الذهبية المقاومة للميثيسيلين باستخدام تفاعل سلسلة البلمرة الهابطة والطرق المظهرية من‬ ‫هدفت هذه الد ارسة الى تقييم انتشار بكتريا المكو ارت العنقودية الذهبية المقاومة للميثيسيلين في بعض المستشفيات الع ارقية‬

Results

Conclusion