Abstract
The sites of transcription initiation for Xenopus laevis mtDNA have been mapped using in vitro capping and primer extension analysis of RNA isolated from oocytes. Transcription of the heavy strand initiates predominantly at a site 33 nucleotides upstream of the tRNAPhe gene. This promoter is bidirectional, with transcription of the light strand initiating only one base pair away. A second, more predominant light strand promoter is located 70 nucleotides away from the major heavy strand promoter. The overall organization of the transcription initiation sites with respect to the tRNAPhe gene and the 5' termini of major stable D-loop DNA strands resembles that of the human mtDNA genome. Analysis of the sequences surrounding the Xenopus start sites suggests that the occurrence of a conserved sequence element, ACPuTTATA, around the start sites may be important for promoter recognition and transcription initiation. This sequence is not found in human mtDNA promoters.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.