Abstract
Three hundred Dolichos bean (Lablab purpureus) genotypes were screened against Dolichos Yellow Mosaic Virus (DYMV) disease. Initial screening was done under field conditions, where disease incidence was calculated for each genotype. Subsequently, selfed progenies of 34 symptomless lines were challenged by sap inoculation under field conditions, out of which only three genotypes, viz. VRSEM-894, VRSEM-887 and VRSEM-860 did not show any symptoms. Using root stalk of susceptible genotype (Ankur Goldy), these three putative symptomless genotypes were further challenged by grafting. The resistant reactions of VRSEM-894, VRSEM-887 and VRSEM-860 were confirmed, as even after 60 days of successful grafting, no viral symptom appeared on all the grafted plants of these genotypes. When subjected to PCR amplification with DYMV coat protein gene specific primer, these three symptomless genotypes did not show any amplification, suggesting that there was no infection of Dolichos Yellow Mosaic Virus in those genotypes.
Highlights
Dolichos bean (Lablab purpureus) is used as a green vegetable, as well as pulse crop, respectively
Most of the diseases have only local importance, but a few diseases make a global impact; one of them is Dolichos yellow mosaic virus (DYMV) disease, which is the most dominant disease of Dolichos bean and caused by the geminivirus, which is transmitted by vector whitefly [4]
The satisfactory control of aphids, mites and partial control of viral disease have been achieved with the application of certain pesticides, which is mostly ineffective, uneconomical, causes environmental hazard and pose a health risk to the farmers and consumers, while an initial pesticide application may be necessary to control heavy infestations; repeated applications may lead to strains of whiteflies that are resistant to pesticides [8]
Summary
Dolichos bean (Lablab purpureus) is used as a green vegetable, as well as pulse crop, respectively. Dolichos yellow mosaic resistant genotypes Pusa Sem-2 and Pusa Sem-3 have been reported previously, under open field conditions [9]. In order to combat DYMV disease as an initiative towards yellow mosaic resistant breeding, germplasm lines were systematically screened, and the results are reported .
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
