Identification of Homologous Polyprenols from Thermophilic Bacteria.

Lucia Gharwalová,Tomáš Řezanka,Irena Kolouchová,Lucie Kyselová,Helena Marešová,Andrea Palyzová

doi:10.3390/microorganisms9061168

Abstract

Sixteen strains of five genera of thermophilic bacteria, i.e., Alicyclobacillus, Brevibacillus, Geobacillus, Meiothermus, and Thermus, were cultivated at a temperature from 42 to 70 °C. Twelve strains were obtained from the Czech Collection of Microorganisms, while four were directly isolated and identified by 16S rRNA gene sequencing from the hot springs of the world-famous Carlsbad spa (Czech Republic). Polyprenol homologs from C40 to C65 as well as free undecaprenol (C55), undecaprenyl phosphate, and undecaprenyl diphosphate were identified by shotgun analysis and RP-HPLC/MS-ESI+ (reverse phase high-performance liquid chromatography–high-resolution positive electrospray ionization mass spectrometry). The limit of detection (50 pM) was determined for individual homologs and free polyprenols and their phosphates. Thus, it has been shown that at least some thermophilic bacteria produce not just the major C55 polyprenol as previously described, but a mixture of homologs.

Highlights

The Eger Graben, called after the river Eger, is a geographic unit in the Czech Republic.It runs SW–NE along the Erzgebirge (Ore Mountains), is part of the European CenozoicRift System, and forms an extensive asymmetric tectonic moat
Its origin is related to the response of Alpine Orogeny in the Bohemian Massif, the manifestation of which was volcanic activity that began in the Late Cretaceous but reached its peak in the Tertiary times
This paper presents two examples of the analysis of polyprenols and their derivatives in thermophilic bacteria, either obtained from the collection of microorganisms (CCM-Brno, Brno, Czech Republic) or directly isolated from the hot springs

Summary

Introduction

The Eger Graben, called after the river Eger, is a geographic unit in the Czech Republic.It runs SW–NE along the Erzgebirge (Ore Mountains), is part of the European CenozoicRift System, and forms an extensive asymmetric tectonic moat. Its origin is related to the response of Alpine Orogeny in the Bohemian Massif, the manifestation of which was volcanic activity that began in the Late Cretaceous but reached its peak in the Tertiary times This volcanic activity was related to the formation of thermal springs in the world-famous. All springs in Carlsbad spa are characterized by a uniform chemistry and are only partial sub-branches from the “Vřídlo” mainstream, which differ in temperature and, depending on it, in the amount of dissolved carbon dioxide. Microbial life in these waters very often remains largely unexplored. Only one microbiological study has been devoted to Carlsbad’s springs, reporting the isolation and characterization of a bacterium of the genus Thermus from the spring “Vřídlo” [2]

Methods

Results

Conclusion