Abstract
Abstract We are studying the role of C-type lectin-like molecules in innate immunity. We have produced recombinant human MINCLE (CLEC4E) protein and generated a specific antibody against this. We find limited expression in a range of immune cells. Human MINCLE co-immunoprecipitates with the FcR gamma chain. Recombinant MINCLE was screened for binding to a variety of glycan arrays and found to bind to several glycolipids, including synthetic glycolipids. Using a quantitative cellular reporter assay, these glycolipids were shown to cause potent cellular activation through MINCLE. Further investigation are underway to assess the relevance of the interactions of MINCLE in human disease. These ligands have great potential as adjuvants.
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