Abstract
The major histocompatibility complex (MHC) in sheep, Ovar-Mhc, remains poorly characterized relative to other domestic animals. However, its basic structure is similar to that of other mammals, comprising class I, II and III regions. In this study, the Ovine MHC class II DRB1 and DRB3 genes were amplified by polymerase chain reaction in eight sheep breeds reared in Turkey. Informative restriction fragment length polymorphisms (RFLPs) were obtained with five restriction enzymes for DRB1 and with two restriction enzymes for DRB3 . The digestion of DRB1 exon 2 with Nci I, Sac I, Sac II, Hin 1I each produced three genotypes and two alleles (viz., a and b) with frequencies of 0.69 and 0.31; 0.65 and 0.35; 0.91 and 0.09; 0.57 and 0.43, respectively. The digestion of DRB1 exon 2 with Dde I produced four genotypes and three alleles (viz., a, b and c) with frequencies of 0.62, 0.28 and 0.10, respectively. On the other hand, the digestion of DRB3 exon 2 with Nde II and Bsa I each produced three genotypes and two alleles (viz., a and b) with frequencies of 0.72 and 0.28; 0.96 and 0.04, respectively. This study presents the genetic profiles of the exon 2 region of the MHC DRB1 and DRB3 genes in native Turkish sheep breeds. Keywords: DRB1, DRB3 , polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP)
Highlights
The major histocompatibility complex is a large genomic region or gene family that is found in most vertebrates, which encodes MHC molecules
Studies of genetic variation in Ovar-Mhc class II genes have shown that the expressed DRB locus is highly polymorphic (Schwaiger & Epplen, 1995; Schwaiger et al, 1996; Jugo & Vicario, 2000; Konnai et al, 2003; Ballingall et al, 2008; Nikbakht et al, 2012; Lotfi et al, 2012; Shen et al, 2014; Takeshima et al, 2014)
In this study, the ovine MHC, class II DRB1 and DRB3 genes were analysed by polymerase chain reaction and restriction fragment length polymorphism (PCR-restriction fragment length polymorphisms (RFLPs)) in eight Turkish sheep breeds
Summary
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