Abstract
The sipunculid worm Sipunculus nudus is a widely distributed, commercially important marine mollusc. Oogenesis and sex determination/differentiation are important steps in S. nudus reproduction. However, the molecular mechanisms of oogenesis and sex determination/differentiation in S. nudus are unclear. We successfully generated a high-quality de novo assembled and annotated transcriptome of oocytes from coelomic fluid (OC), oocytes from nephridium (ON) and spermatozeugmata from coelomic fluid (SC) of S. nudus using the Illumina sequencing platform. There were 17,720 and 17,030 differentially expressed genes (DEGs) in OC vs. ON and OC vs. SC respectively. KEGG pathway analysis showed that the DEGs in these two groups were enriched in ribosome, thyroid hormone synthesis, apelin signalling pathway and cellular senescence. Critical genes that play essential roles in sex determination/differentiation were found, including foxl2, fem-1, dmrt1, ftz-f1 and sox9. This may largely contribute to the understanding of the molecular mechanisms of oogenesis and sex determination/differentiation in S. nudus and laid a foundation for further in-depth studies.
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