Identification of FgDP and FDP by serial dilution protamine sulfate test (SDPST)

Abstract

Listen

Translate article

The identification of FDP, fibrin monomer and FgDP is very important for clinical implication, so that, various methods have been reported. In recent years, the differentiation of intravascular coagulation from primary fibrinolysis has been performed by the laboratory technique based on the paracoagulation phenomena. The fundamental mechanism of paracoagulation is not yet entirely clear, but it may be true that the acidic peptides —split from fibrinogen by thrombin— are related to the phenomenon.In this paper, the authors made a fundamental study on the serial dilution protamine sulfate test (SDPST) reported by Niewiarowski and Gurewich (1971). According to our observations, the test is unaffected by the addition of various amounts of fibrinogen into normal plasma, and is also insensitive to high levels of FgDP added to norml plasma. But, FDP produced in both early and late stages of fibrinolysis gives a positive result on SDPST, even with 5μg/ml of early FDP. Showing a positive result on SDPST by late FDP in normal plasma is not in agreement with the original report. This may be due to residual thrombin activity in reaction mixtures. Fibrin monomer in normal plasma gives a positive result on SDPST. In conclusion, the SDPST assay is sensitive to only FDP and fibrin monomer, and therefore, the positive result of the test indicates the presence of preceded thrombin in vivo. Some clinical data of SDPST is presented and discussed in this paper.