Abstract
烟草是研究植物与病原菌互作的理想材料。鉴定烟草抗病基因及其同源物对揭示抗病机制具有重要意义。近年来公共数据库不断增长的EST序列为烟草表达RGA的鉴定提供丰富的数据。本研究通过拼接GenBank收录的412325条烟草EST序列，获得149606条Uni．EST序列。随后利用已克隆的112个植物R基因蛋白序列对其扫描，检测出1113个NtRGA，其中有273、546、53、102和30个分别包含NBS-LRR、LRR．PK、LRR、PK和Mlo结构域，另有109个未检测到结构域。通过序列比对将1071个NtRGA定位于Ⅳ．benthamiana基因组712个位点上。经搜索，从72个NtRGA中检测出78个SSR，根据其侧翼序列设计64对引物。54对成功从烟草基因组DNA中扩增出清晰条带，9对在24个普通烟草品种间检测出多态性，检出等位基因数2～4个，平均2．56个；41对在6个烟草种间检测出多态性，检出等位基因数2～4个，平均2．61个。
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