Identification of electrophysiological endpoints in stem cell-based systems for developmental neurotoxicity testing.

Abstract

Event Abstract Back to Event Identification of electrophysiological endpoints in stem cell-based systems for developmental neurotoxicity testing. Grzegorz Podrygajlo1, 2*, Samora Okujeni1, 2, 3, Patrick Dini1, 2, 3, Matthew Goddard1, 2 and Ulrich Egert1, 2 1 University of Freiburg, Department of Microsystems Engineering – IMTEK, Germany 2 University of Freiburg, Bernstein Center Freiburg, Germany 3 University of Freiburg, Faculty of Biology, Germany The toxicity of chemicals on brain development is of major concern. A predictive in vitro test for potential developmental neurotoxicity (DNT) needs to be an inexpensive, quick, standardized and predictive alternative to present in vivo methods. Here, we combine endpoints based on attachment, proliferation, differentiation and electrophysiological analyses, comparing four neuronal systems (murine embryonic stem cells, human neural progenitor cells and human teratocarcinoma cells) grown on microelectrode arrays (MEAs). Electrophysiological recordings show a spontaneous electrical activity of the networks derived from murine embryonic stem cells and human teratocarcinoma cells neuronal networks only. Comparison of their properties at different developmental stages shows activity increasing from 1st to 3rd week. Supplementary to the MEAs data we provided measurements of Ca+2 transient curves, which support existence of active neuronal networks in case of murine embryonic stem cells and human teratocarcinoma cells. Moreover, we optimized the differentiation of human teratocarcinoma cells, which now yields up to 2 times more neurons in a 3 to 5 days shorter time. In summary, we analyzed functional endpoints for electrical activity and network properties of 3 cell lines during the period of neuronal maturation. Acknowledgements NT2 cells were obtained from G. Bicker (Tiho, Hannover, Germany) mESC cells were obtained from A. Seiler (BfR, Berlin, Germany) hNPC cells were obtained from E. Fritsche (IUF, Duesseldorf, Germany) Supported by the German BMBF FKZ 0315522B Keywords: developmental toxicity DNT, microelectrode arrays MEAs, networks, Neurons, Stem Cells Conference: BC11 : Computational Neuroscience & Neurotechnology Bernstein Conference & Neurex Annual Meeting 2011, Freiburg, Germany, 4 Oct - 6 Oct, 2011. Presentation Type: Poster Topic: neurons, networks and dynamical systems (please use "neurons, networks and dynamical systems" as keywords) Citation: Podrygajlo G, Okujeni S, Dini P, Goddard M and Egert U (2011). Identification of electrophysiological endpoints in stem cell-based systems for developmental neurotoxicity testing.. Front. Comput. Neurosci. Conference Abstract: BC11 : Computational Neuroscience & Neurotechnology Bernstein Conference & Neurex Annual Meeting 2011. doi: 10.3389/conf.fncom.2011.53.00057 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 23 Aug 2011; Published Online: 04 Oct 2011. * Correspondence: Dr. Grzegorz Podrygajlo, University of Freiburg, Department of Microsystems Engineering – IMTEK, Freiburg, Germany, grzegorz.podrygajlo@imtek.de Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Grzegorz Podrygajlo Samora Okujeni Patrick Dini Matthew Goddard Ulrich Egert Google Grzegorz Podrygajlo Samora Okujeni Patrick Dini Matthew Goddard Ulrich Egert Google Scholar Grzegorz Podrygajlo Samora Okujeni Patrick Dini Matthew Goddard Ulrich Egert PubMed Grzegorz Podrygajlo Samora Okujeni Patrick Dini Matthew Goddard Ulrich Egert Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.