Abstract
BackgroundIt has been found that microRNAs (miRNAs) play important roles in the regulation of tooth development, and most likely increase the complexity of the genetic network, thus lead to greater complexity of teeth. But there has been no research about the key microRNAs associated with tooth morphogenesis based on miRNAs expression profiles. Compared to mice, the pig model has plentiful types of teeth, which is similar with the human dental pattern. Therefore, we used miniature pigs as large-animal models to investigate differentially expressed miRNAs expression during tooth morphogenesis in the early developmental stages of tooth germ.ResultsA custom-designed miRNA microarray with 742 miRNA gene probes was used to analyze the expression profiles of four types of teeth at three stages of tooth development. Of the 591 detectable miRNA transcripts, 212 miRNAs were continuously expressed in all types of tooth germ, but the numbers of miRNA transcript among the four different types of teeth at each embryonic stage were statistically significant differences (p < 0.01). The hierarchical clustering and principal component analysis results suggest that the miRNA expression was globally altered by types and temporal changes. By clustering analysis, we predicted 11 unique miRNA sequences that belong to mir-103 and mir-107, mir-133a and mir-133b, and mir-127 isomiR families. The results of real-time reverse-transcriptase PCR and in situ hybridization experiments revealed that five representative miRNAs may play important roles during different developmental stages of the incisor, canine, biscuspid, and molar, respectively.ConclusionsThe present study indicated that these five miRNAs, including ssc-miR-103 and ssc-miR-107, ssc-miR-133a and ssc-miR-133b, and ssc-miR-127, may play key regulatory roles in different types of teeth during different stages and thus may play critical roles in tooth morphogenesis during early development in miniature pigs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12861-015-0099-0) contains supplementary material, which is available to authorized users.
Highlights
It has been found that microRNAs play important roles in the regulation of tooth development, and most likely increase the complexity of the genetic network, lead to greater complexity of teeth
These results indicate that the chip we designed was able to detect pig miRNAs and suggest that many pig-specific miRNAs may play functional roles in tooth germ development
These results suggest that miRNAs may play an important role in tooth morphogenesis
Summary
It has been found that microRNAs (miRNAs) play important roles in the regulation of tooth development, and most likely increase the complexity of the genetic network, lead to greater complexity of teeth. Many studies have revealed that microRNAs (miRNAs) play important roles in the regulation of tooth development. Conditional deletion of dicer-1 in the epithelium results in mild but significant aberrations in tooth shape and enamel formation [1]. Dicer-1 deletion in another study resulted in multiple and branch enamel-free incisors and cuspless molars, as well as changes in incisor patterning, incisor and molar size and shape [2]. A recent study in mice with a conditional deletion of dicer-1 in the mesenchyme showed an arrest or absence of tooth development between the incisors and molars; extra incisor tooth formation was found in the dicer-1 deletion epithelium [3]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
