Identification of corynebacterium jeikeium by detection of bacterial 16s ribosomal RNA gene

Abstract

Objective To detect clinical strain by amplifying 16S ribosomal RNA(16S rRNA) gene with polymerase chain reaction (PCR) method. Methods 16S rRNA gene was amplifyied by universal primers P1, P2 with polymerase chain reaction, and the polymerase chain reaction products were sequenced to achieve the DNA sequences. Results PCR products were about 1 465bp, and the PCR sequences were blasted in NCBI web to achieve the proper strain name, with the use of this method, the unkown gram-positive bacilli strain was identified as corynebacterium jeikeium strain, ATCC25923 was identified as a strain of staphylococcus aureus and negative control was also specific. Conclusion 16S rRNA gene detection can be used as strain identification, and especially for some less common clinical strains. Key words: Bacteria; RNA, 16S, ribosomal; Polymerase chain reaction