Abstract
The nucleotide sequence of internal transcribed spacer (ITS) region between 18S and 26S ribosomal RNA (rRNA) genes of Oncidium vokelanti cv. 'Strawberry' was amplified by polymerase chain reaction (PCR). The primers for PCR, IT1: 5' CGTAACAAGGTTTCC 3' and IT2: 5' AGTTTCTTCTCCTCC 3' were designed for amplification and sequencing. The length of PCR product was 699 bp. Compare the obtained sequence with the sequence of ITS from other higher plant species, showed that the cloned sequence contained 52 bp of 26S rRNA gene, ITS region and 26 bp of 18S rRNA gene. Thus, the length of ITS region was 621 bp, including 211 bp of ITS1, 163 bp of 5.8S rRNA gene and 247 bp of ITS2. The G+C contents were 54.5, 58.3, and 56.3%, respectively.
Published Version
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