Abstract
Chlorogenic acids are bioactive compounds found in coffee. They are of great interest for their health promoting effects but their bioavailability in human is not fully understood. Hence, it is essential to have strong analytical tools to accurately identify these bioactive compounds. Chlorogenic acids can be cleaved prior to absorption, resulting in the appearance of phenolic acids such as caffeic acid derivatives. A liquid chromatography-mass spectrometry method was developed for the identification of circulating forms of polyphenol-derived metabolites in human plasma after coffee consumption. Separation of metabolites was achieved by reversed-phase chromatography and detection was performed by high resolution MS in negative electrospray ionization mode. A total of 29 coffee metabolites were successfully identified based on co-chromatography (when standard available), mass accuracy and specific fragmentation pattern. Among them, phenolic acid derivatives such as dimethoxycinnamic acid, caffeic acid 3′O-sulfate, dihydroferulic acid appeared to be the main metabolites in human plasma. Only traces of chlorogenic acid conjugates were detected. This method is reliable for the efficient identification of native coffee metabolites in biological fluids. In future, this approach will give more confidence in metabolite identification to better assess coffee polyphenols bioavailability in humans.
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