Abstract
Olfaction plays vital roles in the survival and reproduction of insects. The completion of olfactory recognition requires the participation of various complex protein families. However, little is known about the olfactory-related proteins in Semiothisa cinerearia Bremer et Grey, an important pest of Chinese scholar tree. In this study, we sequenced the antennal transcriptome of S. cinerearia and identified 125 olfactory-related genes, including 25 odorant-binding proteins (OBPs), 15 chemosensory proteins (CSPs), two sensory neuron membrane proteins (SNMPs), 52 odorant receptors (ORs), eight gustatory receptors (GRs) and 23 ionotropic receptors (IRs). BLASTX best hit results and phylogenetic analyses indicated that these genes were most identical to their respective orthologs from Ectropis obliqua. Further quantitative real-time PCR (qRT-PCR) analysis revealed that three ScinOBPs and three ScinORs were highly expressed in male antennae, while seven ScinOBPs and twelve ScinORs were female-specifically expressed. Our study will be useful for the elucidation of olfactory mechanisms in S. cinerearia.
Highlights
Chemoreception, perceived through the olfactory system, plays a critical role in insect behavior [1,2,3]
In the present study, using S. cinerearia antennal transcriptomes, we identified candidate olfactory-related proteins and used quantitative real-time PCR to examine the expression profile of a subset of the transcripts
The antennal cDNA library was constructed from female and male S. cinerearia using the Illumina HiSeqTM platform, and the sequences were assembled by the TRINITY de novo program
Summary
Chemoreception, perceived through the olfactory system, plays a critical role in insect behavior (e.g., seeking hosts, selecting mates, locating oviposition sites, and avoiding adverse environments) [1,2,3]. When the external odorants enter the sensillum lymph, the binding proteins bind to the odorants and transport them to the olfactory receptors on the neuron membrane, in which odorant stimulation is transformed into electric signals. These odorants are degraded by odorant degrading enzymes (ODEs) in the lymphatic cavity or in the sensor cells [5,6,7]. This complex process involves several important families, including odorant-binding proteins (OBPs), chemosensory proteins (CSPs), sensory neuron.
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