Identification of candidate genes involved in marble color pattern formation in genus Salmo

Abstract

Salmonids are known for their variety in skin color and color patterning, which depends on reaction–diffusion mechanism or/and cell–cell interaction. One of the visually most prominent characteristics found in the genus Salmo is the marble color pattern, distinctive for marble trout (Salmo marmoratus). In order to identify and characterize genes potentially involved in skin coloration and color pattern formation in marble trout, a salmonid 32K cDNA microarray was applied to compare skin transcriptome profiles from marble trout, brown trout (Salmo trutta), and marble trout×brown trout hybrids exhibiting the marble color pattern. Microarray results were validated by qRT-PCR and revealed four differentially expressed informative genes (hdac1, vps18, dct and scg2a) involved in animal pigmentation. hdac1 and dct were associated with the wnt signaling pathway, vps18 was involved in melanosome biogenesis and scg2a was observed to act as physiological factor in skin pigmentation and thus could be regarded as a helper protein in prohormone Pomc packaging. We propose that the formation of the marble color pattern is at least partially based upon a reaction–diffusion mechanism and depends upon wnt signaling pathway.