Abstract
Two serotypes of bovine group A rotaviruses were demonstrated by enzyme-linked immunoassay (ELISA) and polyacrylamide gel electrophoresis (PAGE) in 20 of 171 faecal samples collected from diarrhoeic calves in two dairy farms in Venezuela. By serotyping ELISA using G and P serotype-specific monoclonal antibodies, bovine rotaviruses (BRV) circulating on one farm were identified as serotype G6, while BRVs circulating on the other farm were identified as serotype G10. Only one BRV (033) could be successfully isolated in MA104 cells, and the nucleotide sequences of the VP7 and the VP8* trypsin-cleavage product of the VP4 were determined. Cross-neutralization tests and comparative sequence analysis showed that BRV 033 belonged to serotype G6 and genotype P1. This is the first report of BRVs identified in Venezuela.
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