Identification of Bovine Herpesvirus type 5 in nasal swabs of naturally infected calves by Polymerase Chain Reaction

Abstract

In this article, the results of molecular-genetic analyses of nasal swabs are presented. The experimental studies were carried out in the dairy farms of Vologda region suspected to the respiratory infections. The samples were obtained from the asymptomatic calves and from calves with cough and nasal discharges. The laboratory studies were conducted in the laboratory of virology of the FSC VIEV. The genome of the virus was detected by a polymerase chain reaction. The comparative analyses of identified nucleotide sequences of the virus with the reference strains of the International Nucleotide sequence database has shown the similarity with Bovine herpesvirus-5. Accordingly, as a result of our studies, the Bovine herpesvirus type 5 was identified in the nasal swabs of calves in farms of Vologda regions.