Development of differentiation method for bovine herpesvirus serotypes (BHV-1, BHV-4, BHV-5) using polymerase chain reaction

Abstract

Infectious pneumoenteritis of cattle is etiologically caused by viruses of different families and species. Bovine herpesvirus type 1 — infectious rhinotracheitis virus — is the main and the most dangerous pathogen transmitted by cattle semen. At the same time, recently, according to European scientists’ data, in addition to this pathogen, other herpesviruses have been circulating in cattle groups, in particular, bovine herpesvirused of types 4 and 5. Studies have been conducted using molecular-genetic and bioinformatic methods. Based on the analysis of the genomes of bovine herpesvirus of types 1 (IBR virus), 4 and 5 we developed primers BoHV-1 F/R, which flanks the DNA fragment of the IBR virus with the length of 204 bp, BoHV-4 F/R, which flanks the DNA fragment of bovine herpesvirus type 4 with the length of 615 bp, and BoHV-5 F/R for bovine herpesvirus type 5 DNA amplification with the formation of specific fragments 158 bp in length. The tests demonstrated that primers specific for bovine herpesvirus of types 1, 4 and 5 can be used in multiplex amplification format and hybridized only with specific DNA matrices of bovine herpesviruses. A standard operating procedure ‘Indication of DNA of infectious bovine rhinotracheitis virus and bovine herpesviruses of types 4 and 5 by polymerase chain reaction’ has been developed