Abstract
Mulberries are consumed either freshly or as processed fruits and are traditionally used to tackle several diseases, especially type II diabetes. Here, we investigated the metabolite compositions of ripe fruits of both white (Morus alba) and black (Morus nigra) mulberries, using reversed-phase HPLC coupled to high resolution mass spectrometry (LC-MS), and related these to their in vitro antioxidant and α-glucosidase inhibitory activities. Based on accurate masses, fragmentation data, UV/Vis light absorbance spectra and retention times, 35 metabolites, mainly comprising phenolic compounds and amino sugar acids, were identified. While the antioxidant activity was highest in M. nigra, the α-glucosidase inhibitory activities were similar between species. Both bioactivities were mostly resistant to in vitro gastrointestinal digestion. To identify the bioactive compounds, we combined LC-MS with 96-well-format fractionation followed by testing the individual fractions for α-glucosidase inhibition, while compounds responsible for the antioxidant activity were identified using HPLC with an online antioxidant detection system. We thus determined iminosugars and phenolic compounds in both M. alba and M. nigra, and anthocyanins in M. nigra as being the key α-glucosidase inhibitors, while anthocyanins in M. nigra and both phenylpropanoids and flavonols in M. alba were identified as key antioxidants in their ripe berries.
Highlights
Mulberry belongs to the genus Morus, plant family Moraceae, which comprises 24 different species and one subspecies, with at least 100 varieties [1]
Mulberry species have been used in Traditional Chinese Medicines (TCM) for the treatment of several diseases, especially diabetes mellitus type II; they contain specific molecules, i.e., iminosugars or iminocyclitols, which are low molecular weight carbohydrates in which the endocyclic oxygen atom has been replaced by a nitrogen atom
Fourier transformed Mass spectrometer (FTMS) analysis was performed on their aqueous methanol extracts, generating both an LC-photodiode array detector (PDA)
Summary
Mulberry belongs to the genus Morus, plant family Moraceae, which comprises 24 different species and one subspecies, with at least 100 varieties [1]. Mulberry species have been used in Traditional Chinese Medicines (TCM) for the treatment of several diseases, especially diabetes mellitus type II; they contain specific molecules, i.e., iminosugars or iminocyclitols, which are low molecular weight carbohydrates in which the endocyclic oxygen atom has been replaced by a nitrogen atom. These compounds are known to be able to inhibit the enzyme α-glucosidase which is present in the brush border of the human intestine [4,5,6]. Inhibitors of α-glucosidase can prevent the development of diabetes in individuals with impaired glucose tolerance and/or impaired fasting of blood glucose [7]
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