Identification of Bacteria in Kawista Leaves (Limonia acidissima L.)

Jamiatul Aulia Jamiatul Aulia,Nining Purwati Nining Purwati,Sri Sofiati Umami

doi:10.22236/j.bes/515568

Abstract

Background: Bacteria are unicellular prokaryotic microorganisms which are abundant in nature. This study aims to isolate and identify bacteria present in the leaves of Kawista (Limonia acidissima L.). Method: The identification process was carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. The study was conducted at the West Nusa Tenggara Province Testing and Calibration Laboratory for Health, using kawista (Limonia acidissima L.) leaves obtained from West Sumbawa Regency. Results: The results showed eight bacterial isolates were found including Bacillus sp, Bacillus megaterium, Staphylococcus sp, Staphylococcus epidermidis, Staphylococcus capitis, Streptococcus oralis, Leuconostoc mesenteroides spp. mesenteroides, and Shewanella putrefaciens. Conclusion: Diversity of types of bacteria can be studied through the process of identification. This study aims to isolate and identify bacteria found in kawista leaves. The identification method is carried out in a microbiology analyzer with the help of the BD Phonix M50 identification tool. Bacteria found on the leaves of kawista (Limonia acidissima L.) consist of a group of endophytic bacteria, lactic acid bacteria, metal reducing bacteria, and pathogenic bacteria.

Highlights

Kawista (Limonia acidissima L.) is a tropical fruit plant that belongs to Rutaceae family
Metal reducing bacteria group namely Shewanella putrefaciens, whereas the pathogenic bacteria group consists of Staphylococcus sp., Staphylococcus epidermidis, Staphylococcus capitis dan Streptococcus oralis
Eight bacterial isolates were found on Limonia acidissima L. leaves obtained from three dilutions and nine replications

Summary

Introduction

Kawista (Limonia acidissima L.) is a tropical fruit plant that belongs to Rutaceae family. In Indonesia, kawista is widely used by people in Bima district, West Nusa Tenggara as processed food and beverages, and traditional medicines to treat various types of diseases. The kawista plant has not been widely cultivated by the public This fruit is less well known because it is rarely consumed, so that it has almost disappeared on the market. This situation is caused by the lack of literature and reading sources that study the kawista plant. Conclusion: Eight types of bacteria found in the leaves of Limonia acidissima L. consist of a group of endophytic bacteria, lactic acid bacteria, metal-reducing bacteria, and pathogenic bacteria. The metal-reducing bacteria group is Shewanella Putrefaciens while the pathogenic bacteria group consists of Staphylococcus sp., Staphylococcus epidermidis, Staphylococcus capitis and Streptococcus oralis

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