Abstract
A bacterial strain B8S, with nitrogen fixation ability, was isolated from the stalk of sugarcane variety RB867515 introduced from Brazil. This strain was identified based on morphological, physiological and biochemical characteristics and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence analysis showed that the fragment sequence length was 1403bp. When compared withAgrobacterium tumefaciens (AY51349, AB116668.1 and AJ1307119) in GenBank, the homology of the nucleotides was 100%. Certain physiological and biochemical characteristics of new bacterium strain B8S were almost the same as that ofAgrobacterium tumefaciens. Based on its nitrogen fixation ability, the strain B8S was named asAgrobacterium diazotrophicus.
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