Pseudomonas hydrolytica sp. nov., multiple polymer-degrading bacteria isolated from soil in China.

Abstract

A short rod-shaped, Gram-stain-negative strain that can degrade multiple polymers was isolated from forest soil in China and designated as DSWY01T. The results of 16S rRNA gene sequence analysis showed that this isolate shared high similarities with Pseudomonas alcaliphila NBRC 102411T (99.3 %), Pseudomonas mendocina NBRC 14162T (99.2%) and Pseudomonas oleovorans NBRC 13583T (99.0%). The results of phylogenetic analysis based on 16S rRNA gene sequence and multilocus sequence analysis (recA, gyrB, nuoD, glnS and rpoD) indicated that strain DSWY01T belongs to the genus Pseudomonas and is a member of the P. oleovorans group in an independent branch. The average nucleotide identity and digital DNA-DNA hybridization between the genome of strain DSWY01T and the genomes of other species (ANIb 77.72-89.65 %; GGDC 15.50-31.10 %) showed that the isolate represents a novel species. The DNA G+C content of strain DSWY01T was 63.67 mol%, and the major cellular fatty acids (>15 %) were a mixture of C18 : 1ω7c/C18 : 1ω6c and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified lipids, and the major quinone was CQ-10. The morphological, physiological and biochemical characteristics of the isolate were then compared with those of reference type strains. The isolate differed considerably from its closest relatives and is representative of a novel species of Pseudomonas, for which the name Pseudomonas hydrolytica sp. nov. is proposed. The type strain is DSWY01T (=DSM 106702T=CCTCC AB 2018053T).