Abstract
Identification of a marker for two lineages within the GC1 clone of Acinetobacter baumannii
Highlights
Pulsotype I included 52 isolates and was present in the hospital intensive care unit from 1995 to 1999, with a single isolate recovered in 2000
The relationships among these isolates had previously been examined by PFGE of ApaI-digested DNA, determination of MICs of several antibiotics and PCR screening to determine whether the blaOXA-23 gene or class 1 integrons were present.[1]
One or two isolates from each year for each remaining pulsotype were tested using triplex PCRs targeting the oxa-Ab, csuE and ompA genes,[2] to determine whether they belong to global clone 1 (GC1) or global clone 2 (GC2)
Summary
Pulsotype I included 52 isolates and was present in the hospital intensive care unit from 1995 to 1999, with a single isolate recovered in 2000. Isolates from a collection of 177 antibiotic-resistant Acinetobacter recovered at Westmead Hospital, Sydney over the period 1995 – 20021 were further characterized. The relationships among these isolates had previously been examined by PFGE of ApaI-digested DNA, determination of MICs of several antibiotics and PCR screening to determine whether the blaOXA-23 gene or class 1 integrons were present.[1] Eight PFGE pulsotypes were detected.
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