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Abstract
1. 1. Reineckate precipitation and tetraphenylboron separation were performed on acetone/ water extracts of the digestive gland of the marine mollusc Aplysia californica. The isolated choline esters were futher purified and characterized with a variety of chemical methods. The properties of the extracted compounds were compared to those of authentic urocanylcholine and related choline esters of the murexine family. 2. 2. Bioassays were done on identified cholinoceptive neurons of the abdominal ganglion of A. californica using intracellular electrophysiological techniques. 3. 3. The results show that the digestive gland of this animal contains both acetylcholine and urocanylcholine (murexine), the latter accounting for the cholinesterase-resistant cholinomimetic activity of extracts of this organ.
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