Abstract
The expression of a sarcoplasmic reticulum (SR)-like Ca 2+-ATPase was studied in the adult chicken cerebellum. A monoclonal antibody, CaS/Cl-IgG, specific for the cardiac/slow-twitch skeletal muscle SR Ca 2+-ATPase, was used as a probe of protein expression. An immunoblot analysis showed that CaS/Cl-IgG recognized similar size polypeptides in adult chicken heart and cerebellum. CaS/Cl-IgG recognized fragments of similar size after limited tryptic digestion of cardiac and cerebellar membranes. A two-dimensional α-chymotryptic peptide map analysis demonstrated that the cardiac and cerebellar Ca 2+-ATPases were structurally very similar. Immunofluorescence microscopy localized the cerebellar Ca 2+-ATPase to Purkinje cell bodies and dendritic trees. These results suggest that the well-known Ca 2+ uptake system of skeletal and cardiac muscle SR has a remarkably similar counterpart in some neurons.
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