Abstract
A proteomic analysis of the tear fluid suggests that an interplay between proteases and protease inhibitors, and between oxidative reactions, is an important feature of the ocular environment.
Highlights
The tear film is a thin layer of fluid that covers the ocular surface and is involved in lubrication and protection of the eye
Tear fluid was subjected to SDS-PAGE, the gel band cut into 13 slices, in-gel digested with trypsin and the resulting peptide mixtures analyzed by LC MS3 in the linear ion trap-Fourier transform (LTQ-FT)
Our proteomic study highlights the importance of the balance of oxidative reactions, as well as the balance of hydrolase activity and protease inhibitors, as we report here 82 proteins involved in these processes
Summary
The tear film is a thin layer of fluid that covers the ocular surface and is involved in lubrication and protection of the eye. Little is known about the protein composition of tear fluid but its deregulation is associated with disease states, such as diabetic dry eyes. This makes this body fluid an interesting candidate for in-depth proteomic analysis. The eye is covered by a thin, fluid film that serves several functions It has critical roles in the optical system, lubricates the eye, provides nutrients and growth factors to the epithelium and serves as a barrier to the outside environment [1,2]. The function of lysosyme, for example, is to lyse bacterial cell walls
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
