Abstract
Lipoxygenase (LOX) activity was found in the hemolymph of live tiger shrimp (Penaues japonicus Bate) but not in the muscle, branchiae, midgut gland, eye, brain, and spinal cord. Oxygen consumption rate of shrimp hemolymph catalyzed oxidation of arachidonic acid was 1.32 nmol min -1 (mg of protein) -1 . The product was determined to be 12-hydroxyeicosatetraenoic acid (12-HETE) on the basis of retention time in HPLC analysis. The 12-HETE was found to have a conjugated structure on the basis of UV absorbance spectroscopy and to be oxygenated at the C-12 position on the basis of mass spectroscopy and HPLC analysis. The amount of 12-HETE was proportional to oxygen consumption. A 12-lipoxygenase (LOX) activity was identified in the hemolymph of shrimp
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
