Identification, molecular characterization and expression analysis of a mucosal C-type lectin in the eastern oyster, Crassostrea virginica

Abstract

Lectins are well known to actively participate in the defense functions of vertebrates and invertebrates where they play an important role in the recognition of foreign particles. They have also been reported to be involved in other processes requiring carbohydrate–lectin interactions such as symbiosis or fertilization. In this study, we report a novel putative C-type lectin (CvML) from the eastern oyster Crassostrea virginica and we investigated its involvement in oyster physiology. The cDNA of this lectin is 610 bp long encoding for a 161-residue protein. CvML presents a signal peptide and a single carbohydrate recognition domain (CRD) which contains a YPD motif and two putative conserved sites, WID and DCM, for calcium binding. CvML transcripts were expressed in mucocytes lining the epithelium of the digestive gland and the pallial organs (mantle, gills, and labial palps) but were not detected in other tissues including hemocytes. Its expression was significantly up-regulated following starvation or bacterial bath exposure but not after injection of bacteria into oyster's adductor muscle. These results highlight the potential role of CvML in the interactions between oyster and waterborne microorganisms at the pallial interfaces with possible involvement in physiological functions such as particle capture or mucosal immunity.