Abstract
Sugarcane is one of the most important cash crops worldwide, with ability to store high sucrose in its stalk. Due to complex and polyploid genome, improvement in sucrose content in sugarcane through routine genetical and molecular implements is limited. In the era of the next-generation sequencing, transcriptomic studies targeted to different tissues at defined crop stage would play decisive role in identifying distinct transcripts/genes regulating various agronomical traits including sucrose content in sugarcane culm. In the present pursuit, transcriptomes of bottom and top internodes of a high sugar variety (CoJ64) were analyzed to define/validate the differentially expressing transcripts associated with sugar metabolism. Of 173,821 transcripts, 116,777 transcripts differentially expressing in these two sets of tissues were further functionally annotated. Six hundred and eight transcripts were fished out on the basis of their significant read count difference. Ten selected transcripts belonging to sugar synthesis and transportation, cellular signaling, stress responsive genes and transcription factor were validated through real-time PCR, and the results indicated differential expression during stages of sugar accumulation. A specific water stress-related myb transcription factor identified depicted gradual increase in its expression with cane maturity, possibly helping cane to withstand water deficit during ripening stage of the cane for high sucrose accumulation. Beside these some hypothetical and uncharacterized transcripts were also recognized having high expression at immature stage of the crop, presumably enhancing sink strength for high sugar accumulation in this variety supported with the various domains observed in their predicted secondary structure.
Published Version
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