Identification and validation of a major chromosome region for high grain number per spike under meiotic stage water stress in wheat (Triticum aestivum L.).

Ifeyinwa Onyemaobi,Guijun Yan,Hui Liu,Habtamu Ayalew,Kadambot H M Siddique,Meixue Zhou

doi:10.1371/journal.pone.0194075

Abstract

Grain number is a major trait for wheat yield under dryland farming. An International Triticeae Mapping Initiative (ITMI) mapping population comprising 105 recombinant inbred lines (RIL) developed from a cross between a Synthetic hexaploid wheat (Triticum aestivum) ‘W7984’ and a spring wheat variety ‘Opata M85’ was used to identify quantitative trait loci (QTL) associated with grain number per spike under two treatment conditions, normal watering and water stress during meiosis. Two major QTL for grain number per spike on the main stem Q.Gnu.uwa-5A-1 and Q.Gnu.uwa-5A-2 with phenotypic variations of 25.71% and 24.93%, respectively, were detected on the long arm of chromosome 5A when plants were exposed to water stress during meiosis. One QTL (Q.Gnu.uwa-2A) with a LOD score of 2.8 was detected on the long arm of chromosome 2A under normal watering condition. The alleles associated with higher grain number per spike under different treatment conditions came from the Synthetic W7984 parent. Two populations developed from crosses Synthetic W7984 × Lang and Synthetic W7984 × Westonia were used to validate the identified QTL under water stress during meiosis. SSR markers Xbarc230 and Xbarc319 linked with the identified QTL on chromosome 5AL were validated in the two F2:4 segregating populations. These closely linked SSR markers could potentially be utilized in marker-assisted selection to reduce yield loss in regions where water stress during meiosis occurs frequently. The identified QTL can be incorporated into elite lines / cultivars to improve wheat grain yield.

Highlights

Bread wheat (Triticum aestivum L.) is one of the most important food crops consumed by humans [1]
Our research focused on the genetic control and molecular basis of grain number per spike in wheat plants exposed to water stress during meiosis
A recombinant inbred line (RIL) mapping population developed from a cross between the Synthetic W7984 (Altar84/Aegilops tauschii (219) CIGM86.940, female) and Opata (Opata M85, male) [37] along with the parents was used in this quantitative trait loci (QTL) identification study

Summary

Introduction

Bread wheat (Triticum aestivum L.) is one of the most important food crops consumed by humans [1]. It is grown on more than 220 million hectares of land worldwide and provides about 20% of the global daily requirements for calories and proteins [2]. A chromosome region affecting grain number per spike in wheat under water stress. Grain number per spike (GN) and thousand grain weight (TGW) are two of the main components of grain yield in wheat [5]. The study of individual yield components would provide better insight into the genetics of plant development and how it affects yield performance [6,7]. Final grain number determines grain yield in wheat, such that yield increases have been directly associated with increases in grain number [8,9,10,11,12]

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