Identification and quantification of methyl jasmonate in leaf volatiles of Arabidopsis thaliana using solid-phase microextraction in combination with gas chromatography and mass spectrometry.

Abstract

Static headspace sampling with solid-phase microextraction has been used in combination with GC-FID and GC-MS for the specific enrichment, identification and quantification of volatile methyl jasmonate secreted by wounded leaves of Arabidopsis thaliana. The microsample method of analysis was found to be precise, accurate, sensitive and rapid. The detection limit of the procedure is 1.5 ppb (approximately 1.3 ng) per injection, which is of adequate sensitivity to detect the natural baseline levels of methyl jasmonate (approximately 10-100 ng/g) present in plant tissues. The method can be applied to most plants, requires a minimum of sample material, and shows the additional advantage that it is suitable for automation and could thus be used for high-throughput screening.