Abstract
Glioblastoma multiforme(GBM) is the most common brain malignancy. Long non-coding RNAs(lncRNAs) are aber-rantly expressed in many cancers and involved in pathogenesis, progression and metastasis of tumors. In particular, lncRNAs can function as competing endogenous RNAs(ceRNAs). The functional roles of lncRNA associated-ceRNAs in GBM are not fully understood. Human Exon1.0 Microarray(Affymetrix) and Human MicroRNA Microarray(Agilent) were used to detect the expression of 955microRNAs(miRNAs), 33,125lncRNAs, and17,453mRNAs in 8GBM and 8normal brain samples. The function of differential mRNA was determined by Gene Ontology(GO) and pathway analysis. The distinctly expressed miRNAs, lncRNAsand mRNAs were subjected to construct miRNA-lncRNA-mRNA interaction network. The expression of miRNAs, lncRNAsand mRNAs in GBM tissues vs.normal brain tissues was examined by quantitative real-time RT-PCR. A total of 41miRNAs, 398lncRNAs and 1,995mRNAs were found to be differentially expressed between the GBM and normal brain groups. GO and pathway analyses had proven that the functions of differentially expressed mRNAs in GBM related closely with many processes important in the cancer pathogenesis. Fifty-five lncRNAs acting as ceRNAs were identified based on the miRNA-lncRNA-mRNA interaction network. The potential roles of the 39ceRNAs were revealed, which participated in 23diverse cancer biological pathways, including proliferation, cell apoptosis, adhesion, angiogenesis and metastasis. The identified sets of miRNAs, lncRNAs and mRNAs specific to GBM were verified by qRT-PCR experiment in GBM samples. Our study predicts the biological functions of a multitude of lncRNA associated-ceRNAs in GBM. Moreover, our study provides a road map for the identification and analysis of lncRNA acting as ceRNA in tumors.
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