Abstract
BackgroundHost-plant resistance is the most desirable and economic way to overcome BPH damage to rice. As single-gene resistance is easily lost due to the evolution of new BPH biotypes, it is urgent to explore and identify new BPH resistance genes.ResultsIn this study, using F2:3 populations and near-isogenic lines (NILs) derived from crosses between two BPH-resistant Sri Lankan rice cultivars (KOLAYAL and POLIYAL) and a BPH-susceptible cultivar 9311, a new resistance gene Bph33 was fine mapped to a 60-kb region ranging 0.91–0.97 Mb on the short arm of chromosome 4 (4S), which was at least 4 Mb distant from those genes/QTLs (Bph12, Bph15, Bph3, Bph20, QBph4 and QBph4.2) reported before. Seven genes were predicted in this region. Based on sequence and expression analyses, a Leucine Rich Repeat (LRR) family gene (LOC_Os04g02520) was identified as the most possible candidate of Bph33. The gene exhibited continuous and stable resistance from seedling stage to tillering stage, showing both antixenosis and antibiosis effects on BPH.ConclusionThe results of this study will facilitate map-based cloning and marker-assisted selection of the gene.
Highlights
Host-plant resistance is the most desirable and economic way to overcome Brown planthopper (BPH) damage to rice
Segregation of BPH resistance in F2:3 populations To understand the genetic basis of BPH resistance in KOLAYAL and POLIYAL, we investigated the RS segregation at seedling stage in the two F2:3 populations derived from crosses KOLAYAL × 9311 and POLIYAL × 9311
According to the valley bottom, the F2:3 lines could be divided into two classes, the resistant (RS ≤ 6.99) and the susceptible (RS ≥ 7.00), which was consistent with the scoring criterion of BPH resistance (Qiu et al 2010)
Summary
Host-plant resistance is the most desirable and economic way to overcome BPH damage to rice. As single-gene resistance is lost due to the evolution of new BPH biotypes, it is urgent to explore and identify new BPH resistance genes. Spraying insecticides (e.g. imidacloprid) is the chief way to control BPH, which is costly and hazardous to health and environment. It often makes BPH develop resistance to insecticides and leads to resurrection of BPH population (Tanaka et al 2000). Using rice resistance to BPH should be the most economic and effective approach for the management of BPH (Jena et al 2006). More than 30 BPH-resistance genes or quantitative trait loci (QTLs) have been identified from ssp.
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