Abstract
The calmodulin-binding transcription activators (CAMTAs) transcription factor family plays an important role in normal plant growth and development, as well as in biotic and abiotic stress resistance. In this study, we identified seven CAMTA genes across the whole genome of Populus trichocarpa and analyzed the expression patterns of PtCAMTAs in the root and leaf tissues. Promoter cis-element analysis indicated that most CAMTA genes contained stress- or phytohormone-related cis-elements. Quantitative real-time reverse transcription-PCR (qRT-PCR) indicated indicated that PtCAMTAs were induced by mannitol, NaCl, cold stress, pathogenic infection with A. alternata, and phytohormone treatments with abscisic acid, salicylic acid, and methyl jasmonate. We analyzed the expression of homologous genes between P. trichocarpa and P. ussuriensis and alternative splicing forms of PtCAMTA genes under cold stress. We also performed a network interaction analysis for PtCAMTA proteins to predict their interactions and associations. The results of the present study serve as a basis for future functional studies on the Populus CAMTA family.
Highlights
The divalent cation Ca2+, a universal secondary messenger in eukaryotic organisms, is employed by plants
We identified a total of seven calmodulin-binding transcription activators (CAMTAs) genes in P. trichocarpa, which encoded proteins that varied in length from 907 to 1,116 amino acids, with an average length of 1,000 aa
We discovered that PtCAMTA1 and PtCAMTA7 belonged to the same branch of the phylogenetic tree and were present in the homologous regions of chromosomes I and III, respectively
Summary
The divalent cation Ca2+, a universal secondary messenger in eukaryotic organisms, is employed by plants. Several CAMTA genes from different plant species have been characterized, including Arabidopsis, Medicago truncatula, soybean, and maize[6,7,8]. CAMTA transcription factors play an important role in plant growth and development, as well as in biotic and abiotic stress responses, cold stress. Studies indicate that AtCAMTA3 is a positive regulator of CBF2 expression, which is known to be involved in the cold stress response. The majority of MtCAMTA genes have been proven to respond to hormones such as SA, MeJA, and ABA, suggesting that CAMTA-mediated abiotic and biotic stress tolerance may exist in different plant species. The expression mode of PtCAMTAs under abiotic stress (mannitol, NaCl, 4 °C), biotic stress (Alternaria alternate infection), and phytohormone treatment, including abscisic acid (ABA), salicylic acid (SA), and methyl jasmonate (MeJA), were analyzed using quantitative real-time RT-PCR (qRT-PCR). The results may support further functional gene research through the study of these candidate CAMTA genes in response to abiotic and biotic stress
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