Abstract
BackgroundAnu Taila is an ancient medicated oil Ayurvedic preparation that is commonly used for nasya karma. It contains more than 25 herbs and goat milk as per the Ayurvedic Formulary of India (AFI). It strengthens the neck, shoulder, and chest muscles and improves the capacity of sense organs. It delays the aging process and reduces hair fall. Recent studies showed that it is also useful in COVID-19. In the current study, an attempt to develop quality control protocols and evaluate the standardization parameters like refractive index, iodine value, saponification value, peroxide value, acid value, rancidity, HPTLC fingerprint profile along with major bioactive compound and quantification of Berberine chloride, Negundoside, and Marmelosin by HPLC. Establishing quality protocol and standard parameters like physicochemical parameters and estimation of bioactive compounds of this preparation is significant for quality control.ResultsIn this study, HPTLC identifies bioactive chemical compounds like Berberine chloride, Marmelosin, Negundoside, glycyrrhizin, and para hydroxybenzoic acid (PHBA), Lupeol, Embelin, and Solasodine, which were present in the Anu Taila formulation. HPLC was used to estimate the bioactive marker compounds Negundoside, Berberine chloride, and Marmelosin were present in the Anu Taila formulation. The quantitative evaluation of Berberine chloride (0.0013%), Marmelosin (0.0366%), Negundoside (0.0086%) is present in Anu Taila formulation.ConclusionThe study reveals that sufficient quality control parameters were followed during the preparation of the formulation. Physicochemical analysis was carried out as per the guidelines of Ayurvedic Pharmacopeia of India. HPTLC and HPLC profiles generated in this particular study can be considered as a preliminary tool ascertaining the authenticity of Anu Taila.
Highlights
Anu Taila is an ancient medicated oil Ayurvedic preparation that is commonly used for nasya karma
The solvent system was optimized to get the maximum separation of various phytochemicals, and it was further used for the High‐performance thin‐layer chromatography (HPTLC) study
HPTLC identifies some bioactive chemical compounds like Berberine chloride, Marmelosin, Negundoside, glycyrrhizin, and para hydroxybenzoic acid (PHBA), Lupeol, Embelin, and Solasodine, which were present in the Anu Taila formulation
Summary
Anu Taila is an ancient medicated oil Ayurvedic preparation that is commonly used for nasya karma. It contains more than 25 herbs and goat milk as per the Ayurvedic Formulary of India (AFI) It strengthens the neck, shoulder, and chest muscles and improves the capacity of sense organs. (roots), Nelumbo nucifera (flowers), Nymphaea stellata (flowers), Pluchea lanceolata (roots), Santalum album (heartwood), Solanum indicum (whole plant), Solanum surattense (whole plant), Uraria Picta (whole plant), Vetiveria zizanioides (roots), Vitex negundo (seeds), water, goat milk, and all the ingredients are blended in the form of decoction as per the Ayurvedic Formulary of India (AFI) [4, 6] This decoction is slowly infused with sesame oil (Tila taila) over a long time with controlled heating until the oil’s desired quality is obtained. In the recent clinical study, Anu Taila is used as one of the medicines in Ayurvedic treatment regime as nasal drop in the treatment of COVID-19
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