Abstract
In order to select in vitro wheat embryos with a high level of resistance to the fungal pathogen Septoria nodorum (Berk.), extracts from Septoria cultures grown on infected wheat grains were prepared. One of their toxins, mellein, was identified by thin-layer chromatography and its content determined by high-performance liquid chromatography using UV light and fluorescence detection. Synthetic (±)mellein was used as standard. We investigated two techniques of Septoria cultivation, different methods of mellein extraction and the stability of the extracted mellein. In these experiments, the content of mellein in fresh fungal cultures amounted to 46 mg/kg, but in the dry material only 21 mg per kg of dry matter were recoverable.
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