Abstract
Objective To establish qualitative and quantitative analysis methods of cyanidin 3-O-glucoside in Begonia fimbristipla hance. Methods The qualitative method was established with TLC and the quantitive method was established with RP-HPLC. Results The identification method with TLC for cyanndin 3-O-glucoside was specific and reliable. In the assay, the linearity of cyanidin 3-O-glucside ranged from 36 ng to 360 ng (r= 0.999 7, n = 6), and the recovery was 98.7%, with RSD of 2.02%.Conclusions Our detection method is suitable for qualitative and quantitive analysis of cyanidin3-O-glucoside in Begonia fimbristipula hance. Key words: Begonia fimbristipula; Cyanidin 3-O-glucoside; TLC; RP-HPLC; Identification; Assay
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